Danny Welbeck, Alex McCarthy and Danny Rose sat out England training on Tuesday morning as a precaution.
The trio did not take part in the session at St George's Park, with Gareth Southgate preparing his squad for the forthcoming Nations League double-header against Croatia and Spain.
Newcomers Mason Mount, Jadon Sancho, Lewis Dunk and James Maddison were all involved with Chelsea midfielder Ross Barkley back in the ranks for the first time since March 2017.
Southgate's side face Croatia in Rijeka on Friday as they look to exact some revenge for their World Cup semi-final defeat during the summer, with the Nations League clash at Stadion HNK to be played behind closed doors before a trip to face Spain in Seville on Monday evening.
Defenders Luke Shaw and James Tarkowski have already withdrawn from the squad through injury and have been replaced by Dunk and Leicester full-back Ben Chilwell.