Last year's winner Tiger Roll features among 69 horses left in Saturday's Randox Health Grand National at Aintree following the confirmation stage.
Gordon Elliott's popular nine-year-old is on course to attempt to emulate Red Rum in 1974 by completing back-to-back victories in the world's greatest steeplechase.
Tiger Roll could go off one of the shortest-priced favourites in the race's history following a second successive triumph in the Glenfarclas Chase at Cheltenham last month.
Elliott has also left in Outlander, Don Poli, Mala Beach, Dounikos, A Toi Phil, Jury Duty, Noble Endeavor, Monbeg Notorious, Blow By Blow, General Principle, Folsom Blue, Bless The Wings, Out Sam, Borice and Measureofmydreams.
Tiger Roll will now have to carry 11st 5lb after the weights were raised 4lb following the defection of Bristol De Mai.
Heading the handicap is the Tony Martin-trained Anibale Fly, who was fourth last year and finished second in the Cheltenham Gold Cup on his latest start.
Pleasant Company, who failed by only a head to claw back Tiger Roll last year, is among six remaining from the Willie Mullins stable.
Also in the Irish champion trainer's team are Rathvinden, Pairofbrowneyes, Up For Review, Livelovelaugh and Isleofhopendreams.
Other Irish possibles include Pat Kelly's Mall Dini and Jessica Harrington's Magic Of Light and Sandymount Duke.
Heading the home contingent are a pair of greys, the Nick Alexander-trained Lake View Lad and Sue Smith's Vintage Clouds, both owned by Trevor Hemmings.
Among others in the mix are Lucinda Russell's 2017 winner One For Arthur, Mark Bradstock's Step Back, Philip Hobbs' Rock The Kasbah and the Nigel Twiston-Davies-trained Go Conquer.
David Pipe's duo of Ramses De Teillee and Vieux Lion Rouge, dual Topham scorer Ultragold from Colin Tizzard's stable and Robert Walford's Walk In The Mill are also in contention. The Becher Chase winner is now guaranteed a run in the maximum 40-strong field.
Pipe confirmed jockey arrangements for his pair on his official website, www.davidpipe.com.
"David Noonan, who has been aboard Ramses De Teillee in all of his starts this season, will ride him in the big race. David has had one ride in the race previously, finishing 11th aboard Ballynagour in 2017," he said.
"Tom Scudamore will be aboard Vieux Lion Rouge, who will be tackling the contest for the fourth time. He has already won and finished runner-up in the Becher Chase over the unique Aintree fences and has jumped 152 National obstacles with barely a semblance of a mistake.
"Ramses and Vieux Lion Rouge both schooled well over our National style fences last week and are in great form, although they would both appreciate some rain. The weather forecast suggests we may get some - we will just have to wait and see."
Sky Bet, who are paying five places and Non-Runner No Bet on the National, make Tiger Roll their 7/2 market leader ahead of Rathvinden and Anibale Fly at 10/1 and 11/1 respectively.
Tiger Roll will be bidding to become the first back-to-back winner of the great race since the great Red Rum in 1973 and 1974.
Ginger McCain's legendary chaser went on to regain his Aintree crown three years later in 1977.
Full list of entered horses
A maximum of 40 horses will line-up for the race. Here are the current entries, their trainers and allotted weights. Jockeys will follow two days before the race.
Anibale Fly (Tony Martin) 11-10
Valtor (Nicky Henderson) 11-6
Tiger Roll (Gordon Elliott) 11-5
Outlander (Gordon Elliott) 11-4
Don Poli (Gordon Elliott) 11-3
Go Conquer (Nigel Twiston-Davies) 11-3
Mala Beach (Gordon Elliott) 11-2
Minella Rocco (Jonjo O'Neill) 11-1
Lake View Lad (Nick Alexander) 11-1
Pleasant Company (Willie Mullins) 11-1
Ballyoptic (Nigel Twiston Davies) 11-1
Dounikos (Gordon Elliott) 11-00
Rathvinden (Willie Mullins) 11-00
One For Arthur (Lucinda Russell) 11-00
Rock The Kasbah (Philip Hobbs) 10-13
Warriors Tale (Paul Nicholls) 10-13
Regal Encore (Anthony Honeyball) 10-12
Magic Of Light (Jessica Harrington) 10-11
A Toi Phil (Gordon Elliott) 10-11
Jury Duty (Gordon Elliott) 10-11
Noble Endeavour (Gordon Elliott) 10-10
Sandymount Duke (Jessica Harrington) 10-10
Monbeg Notorious (Gordon Elliott) 10-10
Ramses De Teillee (David Pipe) 10-9
Tea For Two (Jane Williams) 10-9
Mall Dini (Patrick Kelly) 10-8
Step Back (Mark Bradstock) 10-7
Ultragold (Colin Tizzard) 10-7
Pairofbrowneyes (Willie Mullins) 10-6
Blow By Blow (Gordon Elliott) 10-6
Up For Review (Willie Mullins) 10-6
Singlefarmpayment (Tom George) 10-6
Vieux Lion Rouge (David Pipe) 10-6
Valseur Lido (Henry de Bromhead) 10-6
Vintage Clouds (Sue Smith) 10-6
General Principle (Gordon Elliott) 10-4
Livelovelaugh (Willie Mullins) 10-4
Walk In The Mill (Robert Walford) 10-4
Folsom Blue (Gordon Elliott) 10-4
Bless The Wings (Gordon Elliott) 10-3
- (Bless The Wings is currently number 40 on the list of entries...)
Captain Redbeard (Stuart Coltherd) 10-3
Joe Farrell (Rebecca Curtis) 10-2
Just A Par (James Moffatt) 10-2
The Young Master (Neil Mulholland) 10-2
Baie Des Iles (Ross O'Sullivan) 10-2
Isleofhopendreams (Willie Mullins) 10-2
Exitas (Phil Middleton) 10-2
Red Infantry (Ian Williams) 10-2
Shantou Village (Neil Mulholland) 10-2
Cogry (Nigel Twiston-Davies) 10-0
Out Sam (Gordon Elliott) 10-0
Fact Of The Matter (Jamie Snowden) 10-0
Mr Diablo (J P Demsey) 9-13
Impulsive Star (Neil Mulholland) 9-13
Kingswell Theatre (Michael Scudamore) 9-13
Carole's Destrier (Neil Mulholland) 9-12
Milansbar (Neil King) 9-12
Borice (Gordon Elliott) 9-11
Measureofmydreams (Gordon Elliott) 9-11
Splash Of Ginge (Nigel Twiston-Davies) 9-11
Ziga Boy (Alan King) 9-11
Highland Lodge (James Moffatt) 9-9
Call It Magic (Ross O'Sullivan) 9-9
Kilcrea Vale (Nicky Henderson) 9-9
Looking Well (Nicky Richards) 9-7
Potters Corner (Christian Williams) 9-6
Morney Wing (Charlie Mann) 9-3
Rathlin Rose (David Pipe) 9-2
Scoir Mear (Thomas Mullins) 9-1