Petra Kvitova has pulled out of the Nature Valley International in Eastbourne because of a hamstring injury.
The world number eight, who won the Nature Valley Classic final in Birmingham and is Sky Bet's 9/2 market leader for Wimbledon glory, was set to play Agnieszka Radwanska in the third round later on Wednesday.
"I'm really disappointed to have to withdraw from Eastbourne this morning," the Czech said in a statement, quoted on the WTA website.
"I felt my hamstring in the Birmingham final and, although I played with tape in my match here yesterday, when I woke up this morning it felt worse. With Wimbledon around the corner I couldn't take the risk of making it worse by playing today."
Kvitova is the eighth seed at Wimbledon, where she will aim for a third singles title.
She added: "With Wimbledon around the corner I couldn't take the risk of making it worse by playing today."