Jeremy Noseda expects to learn more about Gronkowski's capabilities when he lines up in the 32Red Burradon Stakes at Newcastle.
The £100,000 Listed contest takes centre stage on the Good Friday card at Gosforth Park and a strong field of 10 three-year-olds has been declared.
Gronkowski is unbeaten in three starts on the all-weather and was a dominant winner over the course and distance in early February.
However, Noseda acknowledges his charge has far more on his plate tomorrow.
He said: "It's another step up in class for him. This is really going to ask some questions of him.
"He's in great order, I believe in him, but we'll learn a bit on Friday and see where we stand."
The only filly in the field is the Mark Johnston-trained Nyaleti.
The daughter of Arch won the Group Three Princess Margaret Stakes at Ascot last summer but has been off the track since finishing down the field in the Fillies' Mile at Newmarket in October.
Charlie Johnston, assistant to his father, said: "She's good. She's wintered very well and she worked last week and we were very happy with her.
"It looks a warm race, but she's top-rated with the fillies' allowance and this race will give us a good indication of what trip and grade we should be aiming for in the first half of the season.
"She's still in a couple of Guineas' and hopefully that's not unrealistic.
"Looking at her physically, she certainly doesn't look like a sprinter. She's a tall, scopey filly who looks like she wants every inch of a mile, but I know when Ryan (Moore) won on her at Ascot last summer he felt six furlongs was her trip.
"She's going there with a good chance on Friday and going there to win, but at the same time it will tell us a lot about where we're heading."
Another Batt is penalised for winning a Group Three race in Turkey last September and as a result must concede weight all round on his first start since.
Trainer George Scott said: "He's in great form, he's fit and ready to go and we're looking forward to getting him started again.
"I think he's improved physically over the winter and we're very happy with him at home.
"He has to give the penalty away to some very good horses, so it's going to be a tough task and we've got to be realistic.
"However, it will tell us a lot about what class he should be in and also what distance he should be running over.
"It's not about tomorrow. This is just a starting point to what we hope will be a very successful and lucrative season."
The sole Irish challenger in the field is Freescape from David Marnane's yard.
The chestnut gelding is fit from a spell in Dubai and was last seen finishing third in a Listed contest at Meydan at the start of the month.
"He's fit and he's well. He was in good nick coming off the plane so we decided we'd have another go," said Marnane.
"It's a big pot, he's a horse we like and we've pencilled him in for the Britannia at Royal Ascot.
"He's won on the all-weather surface at Dundalk. He did that really well so we're hoping he can put up a good show."
Other contenders include John Gosden's Purser and the Richard Fahey-trained Great Prospector.