Colombia boss Jose Pekerman has stated that he is "extremely concerned" by the injury picked up by star man James Rodriguez in the victory over Senegal.
The Bayern Munich midfielder was forced off after half an hour in their final Group H clash - a win that saw the South Americans top the group.
The result means they face England in the round of 16. Gareth Southgate's much-changed side lost to Belgium on Thursday night.
Speaking after the game, Pekerman said that he was worried about the condition of the 26-year-old, who could be a doubt for their knockout stage tie.
"I'm extremely concerned, it's a very tough situation for my team," he said.
"I didn't want to talk about this in a conference, because it risks overshadowing everything else that's happened. But I can say it's not a comfortable situation for us."
Rodriguez was restricted to a cameo appearance in Colombia's opening game, a 2-1 defeat to Japan, because of a minor calf injury, before starting the win over Poland and again against Senegal.
When asked if Rodriguez had suffered a fresh injury, Pekerman said: "At the moment we don't have information, I can't confirm or deny.
"He trained normally until yesterday as always, he stayed on afterwards to practise free kicks and penalties as he does.
"He was fully fit... but right now I don't know where he stands. We do hope we'll hear good news once he's gone for his check-up."