Former British number two James Ward fell at the final hurdle in his bid to qualify for Wimbledon.
The 31-year-old, a Davis Cup winner in 2015, was beaten in four sets by Frenchman Benjamin Bonzi in the final qualifying round at Roehampton.
Ward, who is trying to work his way back up the rankings after two years of knee problems - he is currently ranked 362 having been as high as 89 - broke Bonzi in the first game of the match.
Yet Bonzi quickly redressed the balance and reeled off the first two sets before Ward rallied to take the third.
However, that was as good as it got for the Londoner, who was eventually beaten 6-4 6-2 1-6 6-0 by the world number 198 and missed out on a minimum £39,000 pay day.
Ward's defeat meant no British players made it through the qualifiers. Britain's entrants in the main draw will be Andy Murray - if he is fit enough - Kyle Edmund, Cameron Norrie, Johanna Konta, Heather Watson and wild cards Jay Clarke, Liam Broady, Naomi Broady, Katie Boulter, Harriet Dart, Katy Dunne, Katie Swan and Gabriella Taylor.