David Humphreys will leave his position as Gloucester director of rugby at the end of June, the club has announced.
It follows the exit of head coach Johan Ackermann last month.
Former Ireland fly-half Humphreys, 48, arrived at Kingsholm in 2014 having spent the six previous years coaching at Ulster.
Gloucester won the European Rugby Challenge Cup in his first season and finished third in the Gallagher Premiership in 2019.
But the club had won only four of their 13 fixtures this term when the campaign was halted by the coronavirus pandemic.
"It has been a huge honour to have played a small part in the history of this great club. I have loved every minute of my time here and have been very fortunate to work with great coaches and support staff, and with great players," said Humphreys.
"However, following Johan Ackermann’s departure last month, I took some time to review my own position at Gloucester and in light of many new challenges facing professional rugby as a result of Covid-19, I felt it was the right time for me to move on and a new head coach to come in and build on the work that has been done.
"It is always difficult to leave somewhere that has become home and I will always be very grateful to everyone in the west country for the very warm welcome they gave to both me and my family."