Southampton left-back Ryan Bertrand has pulled out of the England squad due to a back issue.
The 28-year-old was named in Gareth Southgate's 27-man squad for the World Cup warm-up friendlies against Holland and Italy.
Bertrand helped Southampton to a 2-0 FA Cup quarter-final victory against Wigan on Sunday and has now returned to the club as a precautionary measure.
A Football Association statement read: "Southampton defender Ryan Bertrand has returned to his club for further assessment on a back issue.
"Bertrand's withdrawal is a precautionary measure and he will play no part in the forthcoming fixtures against the Netherlands and Italy."
Ashley Young and Danny Rose remain left-back options in the England squad.
Meanwhile, Liverpool right-back Trent Alexander-Arnold was drafted into England training on Tuesday.
To Make England World Cup 2018 Squad (odds via Sky Bet)
- Kyle Walker - 1/12
- Kieran Trippier - 1/4
- Trent Alexander-Arnold - 8/1
- Jonjoe Kenny - 10/1
- Nathaniel Clyne - 10/1
Southgate brought England Under-21 defender Alexander-Arnold into the session to help bolster the numbers, with the teenager taking part in an attack versus defence drill towards the end of the session.
The 19-year-old has regularly rotated at Liverpool with England defender Joe Gomez this season, making 22 appearances in all competitions.
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