Chelsea defender Fikayo Tomori has been handed a first England call-up as team-mate Tammy Abraham also returns to Gareth Southgate's squad.
The Three Lions travel to take on the Czech Republic in Prague on October 11 before a trip to face Bulgaria three days later.
Tomori and Abraham were two of three players included this time around who had missed out on the previous squad, with Everton midfielder Fabian Delph also recalled.
Manchester United pair Jesse Lingard and Aaron Wan-Bissaka drop out, along with Alex Oxlade-Chamberlain of Liverpool.
Tomori, who was born in Canada and represented them at Under-20 level, has established himself in Frank Lampard's Chelsea side of late and is rewarded with a maiden senior England call-up.
Abraham scored his eighth goal in 10 games in Chelsea's 2-1 Champions League win over Lille on Wednesday night and is back in the squad having made his debut almost two years ago.
He featured in friendly matches against Germany and Brazil in November 2017 and remains eligible for Nigeria unless he picks up a competitive cap in the forthcoming games.
Manchester City right-back Kyle Walker is again a high-profile absentee, with Kieran Trippier and Trent Alexander-Arnold preferred.
Tammy Abraham (Chelsea), Trent Alexander-Arnold (Liverpool), Ross Barkley (Chelsea), Ben Chilwell (Leicester City), Fabian Delph (Everton), Joe Gomez (Liverpool), Tom Heaton (Aston Villa), Jordan Henderson (Liverpool), Harry Kane (Tottenham Hotspur), Michael Keane (Everton), James Maddison(Leicester City), Harry Maguire (Manchester United), Tyrone Mings (Aston Villa), Mason Mount (Chelsea), Jordan Pickford (Everton), Nick Pope (Burnley), Marcus Rashford (Manchester United), Declan Rice(West Ham United), Danny Rose (Tottenham Hotspur), Jadon Sancho (Borussia Dortmund), Raheem Sterling (Manchester City), Fikayo Tomori (Chelsea), Kieran Trippier (Atletico Madrid), Callum Wilson(AFC Bournemouth), Harry Winks (Tottenham Hotspur)
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