Key all-rounder Stokes suffered a tight left hamstring during fielding practice the previous day and was then unable to bowl in the nets.
He sat out England's squad football match early on Thursday - while the uncapped Sam Curran, brought in as cover for Stokes, took a full part.
Stokes instead undertook shuttle runs with England's physio on the outfield, having reported a slight improvement in the injury overnight.
England were hoping he would be able to bowl later.
Stokes will have a scan on his hamstring injury this afternoon.
The Durham all-rounder is not expected to bowl or field today, placing his involvement in the second and deciding Test against Pakistan in some doubt.
England will wait until the outcome of a scan on Stokes' hamstring before naming their team for the second NatWest Test against Pakistan.
"Until we know how serious it is it's hard to make decisions and because of that we can't name a team today," England captain Joe Root told Sky Sports News.
"Of course if Ben's fit we want him in the side, he's one of our best players, he brings a lot to the table and offers so much to this team."
Pakistan won the first Test by nine wickets at Lord's last week and Root believes a quick return to action will benefit his side.
"I think it helps us getting straight back into it, not having too much time to think about things," he added.
"It was very clear where we needed to improve from last week. We've had some good preparation, the guys have really worked hard and now it's just doing it, going out and proving a point, putting a really strong performance in as a group and showing some pride in the badge."
Lancashire opener Keaton Jennings returns to the side to partner Alastair Cook at the top of the order and Root added: "He will be desperate to take his opportunity. Every player in this side wants to make an impact this week.
"Every time we play a Test match there are all sorts of different pressures that come with it and for Keaton it's a great opportunity. I hope he looks at it that way and goes out and expresses himself in his own way."
Asked if he was feeling the pressure of arresting England's poor run of form, Root said: "When you are winning and performing well you have to keep churning out those performances and when you're on the other side of it you've got to make sure you turn it round.
"As always I've gone into practice, felt if there were any areas I wanted to work on and got them done so I can go in full of confidence tomorrow and know I've given myself the best chance. It's nice to have that feeling going into any game."
Sky Bet make England 4/7 favourites with Pakistan 100/30 and the draw on offer at 4/1.
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