Chris Woakes has joined Ben Stokes on the injury sidelines for England's first one-day international of the summer against Scotland next weekend.
Woakes, who will be replaced by Tom Curran in the squad to play in Edinburgh, left the field late in England's series-levelling Test match win over Pakistan at Headingley, with a thigh injury.
The England and Wales Cricket Board then confirmed via its Twitter account early on Monday morning that the seamer will not be travelling for Sunday's one-off match.
A brief ECB statement read: "Chris Woakes has been ruled out of the Scotland ODI with tightness in his right quad.
"He is replaced in the squad by Surrey's Tom Curran.
"Woakes will be assessed this week to ascertain if he'll be fit for the Australia series."
Woakes' participation in the five-match Royal London Series against Australia is therefore uncertain - as is Stokes', after he missed the Leeds Test with a hamstring tear.
Curran has eight ODI caps and was part of the team which won world number one England's last series in New Zealand three months ago.
He was not chosen initially to face either Scotland or Australia, however, following Liam Plunkett's return to fitness.