Jos Buttler and Dom Bess have been selected for the first Test against Pakistan but James Vince and Moeen Ali both miss out.
Buttler has been recalled following a stellar run of form in the IPL for Rajasthan Royals and will bat at number seven with Jonny Bairstow moving up to five and keeping the gloves.
Buttler's return surely spells the end of James Vince's international career despite the Hampshire man making a double century in the last round of County Championship matches.
Vince, 27, was recalled to the side at the beginning of last winter and once again displayed considerable promise as he made 336 runs at 30.54.
However, he is still without a Test hundred and his continued failings outside off stump appear to have made up the minds of selectors.
His omission leaves the troublesome number three spot open and national selector Ed Smith has confirmed that Root will now bat at first-drop.
Jack Leach was expected to fill the spinners' spot he held in the final Test in New Zealand in March but a broken thumb picked up while playing for Somerset had seemingly paved the way for Moeen Ali to return.
The selectors have opted to go with a younger man, though, with Bess getting the nod.
Bess, like Leach, has proved a vital cog in Somerset's attack in the last year or so and despite only taking one championship wicket so far this term, England clearly view him as a man with a big future. He will compete with Mark Wood and Chris Woakes for final the bowling spot.
There is a strong possibility that England could field at five-man seam attack at Lord's, with Bess missing out, though a warm forecast could push England into picking a spin option.
Smith said: "Jos Buttler is an outstanding talent, who is already a central part of England's white ball teams.
"The selection panel feels that this is the perfect moment to reintroduce Jos to Test cricket, where he has already enjoyed some success, including a stint playing as a specialist batsman at number seven.
"Jos is playing with great confidence and flair, and he will bring unique qualities to the Test team."
Smith added: "Dom Bess has made an excellent start to his first-class career
"He has impressed everyone with his performances for Somerset and when he has been given opportunities with England representative teams.
"With Jack Leach missing out due to injury, the selection panel wanted to invest opportunity in a young spin bowler.
"Dom's strong form, character and all-round abilities presented a compelling case for selection."