England have ruled bowler James Anderson out of the rest of the Ashes series with Australia having failed to get over his on-going calf injury.
Anderson had been bowling for Lancashire second team when aiming at a return to the Ashes for the fourth Test at his home ground of Old Trafford starting on Wednesday.
However, the calf injury that forced him out early on in the opening Test of the series has cropped up again in the aftermath of his return to bowling, and it is serious enough for the ECB to rule him out for the remainder of the contest with Australia.
Somerset seamer Craig Overton has been called-up into England's 13-man squad as cover, having taken 32 wickets in eight County Championship matches this season at an average of 21.34.
Injured calf plagues Anderson
The injury has plagued Anderson for a number of weeks, with England's record wicket-taker feeling pain in the area as he sought to prove his fitness in a Lancashire Second XI fixture earlier this week.
Anderson will therefore miss out at his home ground of Old Trafford for the Test which gets under way on September 4, with Overton added to the 12 that was named ahead of last week's dramatic one-wicket victory at Headingley.
There has been some scrutiny on Jason Roy, whose paltry average from seven innings as a Test opener stands at 8.85, but he has been retained, meaning Surrey team-mate Ollie Pope - tipped for a recall - misses out.
Anderson suffered an initial injury to his calf during a County Championship fixture against Durham in June, placing his involvement in the first Test in some doubt.
While he managed to take to the field, another injury to the same area restricted him to four overs on the opening morning at Edgbaston and precluded his involvement for the second and third Tests.
He stepped up his recovery by bowling 20 overs on Tuesday while representing Lancashire in a second-string fixture against Durham at Chester Boughton Hall and sent down a further nine on Thursday.
But it was at the conclusion of his nine-over spell where he became aware of more pain to his calf, and after consultation with the England medical team, he was made unavailable for the rest of the Ashes.
Meanwhile, it what could be a swing of momentum even when the two sides are not facing each other, batting talisman Steve Smith was back at the crease for Australia in their tour match against Derbyshire.
Smith missed the defeat at Headingley but his return, coupled with the continued absence of Anderson, would swing the balance back in Australia's favour despite their morale-sapping defeat in Leeds.