No Nay Never set a new two-year-old course record at Ascot when taking the Norfolk Stakes, the opening event on Ladies' Day at the Royal meeting, under Joel Rosario.
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The American raider, giving Wesley Ward his third success at Royal Ascot after two winners in 2009, missed the break and was last after the first 100 yards.
But the well-backed 4/1 chance quickly cut through the pack to take up the lead with Ambiance after little more than a furlong, and although he was challenged and clearly headed with a furlong and a half left to run, but Rosario asked him to quicken again and No Nay Never picked up to score from 9/4 favourite Coach House by a length.
Wind Fire (16/1), the only filly in the contest, stayed on late to finish third just ahead of Ambiance.
"He broke slowly on his first start and he broke slowly again, but he came through for us," said Ward, who had made clear his belief in No Nay Never in the build-up to the race.
"We put a lof of time and effort into this. My staff at home do a lot of work, we start breaking them in early in November and they never miss a day.
"The people at Keeneland did us a favour by letting us breeze on the grass in preparation for this and I want to thank them very much.
"I'm not used to dressing up like this, in fact I don't wear a suit very often, but I don't want wearing them if we get days like this."
Told that his horse had taken three-tenths of a second off the previous course record, he said: "That's what we expected."
No Nay Never will now be aimed at Deauville's Prix Morny, according to Ward, although he will travel back to the US first this weekend.