Reckless Abandon is reported to be lame and will miss Saturday's Betfred Sprint Cup at Haydock.
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Clive Cox's three-year-old colt has been restricted to just two runs this term, having finished third in the Temple Stakes on his reappearance at Haydock and fifth in the King's Stand Stakes at Royal Ascot.
He had gone through his juvenile campaign unbeaten in five races, culminating in Group One wins in the Prix Morny and Middle Park Stakes.
Cox still has the favourite in the six-furlong feature on Merseyside in Lethal Force, winner of the Diamond Jubilee Stakes and July Cup this summer.
Cox revealed Reckless Abandon was found to have a hamstring problem. "It's a big shame, but unfortunately he's not 100% and he can't be there," he said.
"He's not 100% sound and he's sore in his hamstring which is the reason for him not being sound.
"Although we've worked on it as hard as we can this morning, we've had to admit defeat. He pulled out stiff this morning and he's just not 100% to perform tomorrow."
However, Cox reports Lethal Force to be ready for action. His Diamond Jubilee and July Cup hero bids to regain winning ways after playing second fiddle behind Moonlight Cloud a month ago.
"Lethal Force is in excellent form and I really hope conditions are suitable for him. I couldn't be happier with him," said the Lambourn trainer.
"He's in great nick and obviously he has already proved this year he's at the top of his game. Hopefully he'll fly the flag for us.
"He came back very well indeed from his run in France. He's very full of himself.
"That was a very good race. She's (Moonlight Cloud) a very special filly. With her being on home territory it was a tough nut to crack and it was a course record.
"Adam (Kirby) knows our horse really well and his impression on the day was that he probably wasn't quite as sharp as he was for the July Cup run."
Gordon Lord Byron was three-quarters of a length behind Lethal Force when third at Deauville and he was second in this race 12 months ago.
However, he goes to Haydock on the back of a Group Three success over a mile at Leopardstown and his trainer Tom Hogan is full of hope.
"He's in very good form. He arrived over there on Thursday night and was out on the track on Friday morning. He's in very good order," said the County Tipperary handler.
"The rain is fine, but a drop was needed as it would have been very quick if they didn't get a drop.
"Last year we thought we were drawn on the side with pace and it didn't work out like that. Hopefully this year we will be.
"We were catching Lethal Force in Deauville the last day we met, so hopefully there will be a good pace and we might get by him one of these days.
"He improved last backend so hopefully he will improve at the same time of year again."
Richard Fahey feels that significant rain would aid Garswood's cause as his talented three-year-old colt is tackling a shorter trip after winning the Group Two Lennox Stakes at Glorious Goodwood.
"The more rain the better to slow the others down a bit because we're dropping him back in trip. Slowing down the race a bit would help us," said the Malton handler.
"He's in great form and we're delighted with him. He goes there in top shape. He's got to step up.
"We switched him off this year to try to get a mile and he has won over seven. The ground is not a huge issue, it's just to slow them down and the better chance we'll have."
Trainer Eddie Lynam has his fingers crossed Slade Power does not repeat his antics in the stalls that cost him his chance in the Nunthorpe Stakes at York.
The four-year-old blotted his copybook after a series of good efforts that included a third place behind Lethal Force in the July Cup.
"He's in good form, I just hope he behaves himself in the stalls and has a good run. He was a bit naughty in the gates the last day," said the County Meath handler.
"He'll be fine with the track, but we seem to be drawn away from the pace and there's nothing we can do about that.
"Unfortunately it has been raining there. He has won on soft ground. In an ideal world I'd like the ground to be good."
Hawkeyethenoo has been a decent yardstick in the sprinting division for several seasons and trainer Jim Goldie is hoping the seven-year-old can run up to scratch despite starting from what could be an awkward draw (16).
"He won't mind a wee bit of rain, but not that much," said the Glasgow handler.
"We wouldn't have picked that draw, but hopefully something will go forward on our side of the track as Lethal Force is in one.
"He was unlucky at Goodwood but he's an obvious player if he runs to that sort of form again."
The more rain the better for Godolphin's representative Soul, who has some solid form when he can get his toe in.
"The key for him is he needs softer ground. In the morning when he works on firm ground he does not work well, but he shows his best form when it is soft," said trainer Saeed bin Suroor.
"Six furlongs is best for him, as he showed when he was fourth behind Black Caviar at Ascot. He is doing well and any rain will help."
The Mike de Kock-trained Kavanagh carries South African hopes on the back of a fair effort at Newmarket on his first start for three months.
"Kavanagh is very capable on his day. That last run brought him on nicely and he looks a happy horse. He's up against some good opposition but should run well," de Kock's assistant Steven Jell told mikedekockracing.com.
William Haggas believes his two runners Heeraat and Rex Imperator go there with each-way claims.
Both have recorded notable successes this season with Heeraat taking the Group Three Hackwood Stakes at Newbury and Rex Imperator the Stewards' Cup at Glorious Goodwood.
"They are both well and I'm happy with them. I gather it's rained there so I think it will suit Heeraat well," said the Newmarket trainer.
"The race at York was a bit of mess last time for Rex Imperator (second to Sirius Prospect over seven furlongs).
"A fast gallop over six is obviously what makes him tick. He goes on the ground but whether he is quite as good on the ground, I don't know. He'll run OK.
"I think they've got sporting each-way chances."
The persistent rain could put into question the participation of Kingsgate Native.
The eight-year-old was a a course winner in the five-furlong Temple Stakes in May and was fifth behind his stablemate Jwala in the Nunthorpe Stakes at York.
"He's in tip-top condition and ready for action, but the rain does concern me," said trainer Robert Cowell.
"He'd probably take his chance on good to soft as it was good, good to soft at York.
"If it got an awful lot softer than that then I think we may have to reconsider."
(reopens) Kingsgate Native was later declared a non-runner due to the deteriorating conditions.