Wigan chairman Dave Whelan wants new charges laid against West Ham, while admitting it is impossible to overturn the original decision on Carlos Tevez.
Whelan revealed he has held talks with Sheffield United on Monday - and told them "to get into the bones of the Premier League, and quickly".
The Blades were relegated from the top flight on Sunday following defeat at home by the Latics - with West Ham surviving following a 1-0 victory at Manchester United, the winning goal coming from, ironically, Tevez.
However, the south Yorkshire club are set to pursue the possibility of a legal challenge against the Premier League as they look at challenging West Ham's right to play Tevez and the ruling of the league's independent commission.
A record £5.5million fine was handed out for irregularities over the signings of Argentinians Tevez and Javier Mascherano when many thought they should have had a points deduction, which would have probably resulted in the Hammers and not Sheffield United being relegated.
With the saga set to drag on, Whelan is predicting the Blades have got a strong case for new charges.
"We cannot reverse the decision the commission came out with," said the Wigan chairman, who today announced Chris Hutchings as the club's new manager following the shock departure of Paul Jewell.
"It is impossible - they we appointed by the Premier League and all the clubs agreed these people would take charge of the case.
"But now we are saying he [Tevez] has been registered illegally for a second time. Who owns him? Where will this £30million be going we are told he is now going for?"
Whelan insisted: "We have to clean all this up. Time is on the side of the Premier League, I have said to Sheffield United that they must get stuck into the League's bones in the next seven days.
"Sheffield United have told me they have a fantastically strong case.
"We need a new hearing because he has been registered illegally again. That should be another fine or points deduction."
Backing for the so-called 'Gang of Four' - Sheffield United, Wigan, Fulham and Charlton - is growing.
Middlesbrough - according to Whelan - are the latest club reported to be prepared to back the challenge and there are others believed to be waiting in the wings.
Whelan declared Premier League chief executive Richard Scudamore "has got a lot of explaining to do".
The Wigan chairman revealed more could be made clear over the next 24 hours, with the Premier League faxing all member clubs with further details of clarification.
Whelan added: "I have had a fax off him today telling me that all Premier League clubs are going to get an explanation tomorrow."
The Wigan chairman added: "The West Ham situation is in the hands of Sheffield United, we fully support them. Fulham do, Charlton do, Middlesbrough do.
"All those clubs are committed to helping Sheffield United to fight this case.
"I do not think we can reverse the original decision. We have to accept that - but what we can look at is how they opened a new transfer window to sign an illegal player.
"He was illegal, they were fined £5.5million for playing an illegal player all season. Then they open a new window from a Friday at 4pm and leave it open until midday the following day to register him and play against Wigan.
"When [Javier] Mascherano went to Liverpool, it took five weeks to get clearance - and they used the same contract."
Whelan added: "Now we are told Tevez is on the market for £30million. West Ham will not get a penny of that, so who owns him?
"Who owns him now? West Ham do not, they will not get any of the money.
"I cannot believe the Premier League will allow these things to happen and put Sheffield United into the Championship and leave West Ham in, it is not right, not fair."
Sheffield United plc chairman Kevin McCabe is planning to canvas support from fellow top-flight clubs over the possibility of a legal challenge.
McCabe is to take the campaign to the clubs as he felt there was a groundswell of support.
"It needs conversations with respective chairman and directors for them to understand all of the injustices but most of them do know that already," said McCabe.
"I think there is a consensus most clubs support an injustice so I hope we will get the vast majority of clubs in the Premier League supporting our case."
Even so, West Ham chairman Eggert Magnusson remains confident the original ruling will be upheld.
"The matter is in the hands of the Premier League and I am content to leave it so," the Icelander said.
"The Premier League are clear that they are satisfied with things and as far as I am concerned that is that.
"I always wanted our destiny to be decided out on the pitch - and it has been."